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Using reverberators to modify space acoustics
Pavlikovský, Vladislav ; Orlovský, Kristián (referee) ; Kurc, David (advisor)
This diploma thesis deals with adjusting the reverberation time of enclosed spaces. It is divided into two thematic areas. The first thematic area deals with active systems that adjust the reverberation time, with a stronger focus on usage of reverberators to simulate secondary spaces. The second thematic area is the implementation of reverberators and their fundamental building blocks in Matlab.
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Significance, occurrence and determinants of horizontally transmissible colistin resistance in Gram negative bacteria
Kislíková, Karolína ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
Colistin, also known as polymyxin E, is antibiotics active against most of Gram-negative bacteria. In the pas decade, emergency of multidrug-resistant bacteria led to increase of colistin administration as a last resort antibiotic for human infections. The first plasmid-mediated colistin resistance gene mcr-1 was identified in 2015 in animals in China and after first detection, additional mcr genes: mcr-2, mcr-3, mcr-4, mcr-5, mcr-6, mcr-7 a mcr-8 were described throughout the world. The aim of this thesis was to clarify whether there is horizontal transmission colistin resistance encoded by the mcr genes in gram-negative bacteria isolated from the environment, animals and their breeding and food. The mcr-1 gene was detected in 2 strains Escherichia coli isolated from waste water. The mcr-4 gene was detected in 1 strain Shewanella putrefaciens isolate obtained from the lake. The environment is the most important source and way of spreading this type of resistance in the Czech Republic.
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Colistin resistance in clinically important Enterobacteriaceae
Smělíková, Eva ; Tkadlec, Jan (advisor) ; Ježek, Petr (referee)
Colistin is a last-resort antibiotic used to treat serious infections caused by Enterobacteriaceae and other multidrug resistant gram-negative bacteria. Recently discovered plasmid-borne colistin resistance, mediated by the mcr genes, poses a serious risk to colistin therapy. The aim of this diploma thesis was to map the occurrence of Enterobacteriaceae carrying the mcr-1 to 8 genes in hospitalized patients, travellers, prospective colistin-resistant clinical isolates and in a retrospective collection of Enterobacteriaceae using a combination of selective cultivation and qPCR. Isolates with a detected mcr gene were characterized by Whole-Genome Sequencing. The localization of mcr genes was determined and other resistance genes and plasmids were identified. Furthermore, the physiological profile of selected colistin- resistant Escherichia coli isolates was characterized. In the presence of a subinhibitory amount of colistin, a strain carrying the mcr-1 gene may be favored. Later, the mcr-9 gene was described and its occurence was subsequently tested retrospectively. Enterobacter spp. isolates carrying the mcr-9 gene were mostly colistin-sensitive but, in some cases, resistance was induced after exposure to sublethal doses of colistin. The results of the study show that the incidence of plasmid-mediated...
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Significance, occurrence and determinants of horizontally transmissible colistin resistance in Gram negative bacteria
Kislíková, Karolína ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
Colistin, also known as polymyxin E, is antibiotics active against most of Gram-negative bacteria. In the pas decade, emergency of multidrug-resistant bacteria led to increase of colistin administration as a last resort antibiotic for human infections. The first plasmid-mediated colistin resistance gene mcr-1 was identified in 2015 in animals in China and after first detection, additional mcr genes: mcr-2, mcr-3, mcr-4, mcr-5, mcr-6, mcr-7 a mcr-8 were described throughout the world. The aim of this thesis was to clarify whether there is horizontal transmission colistin resistance encoded by the mcr genes in gram-negative bacteria isolated from the environment, animals and their breeding and food. The mcr-1 gene was detected in 2 strains Escherichia coli isolated from waste water. The mcr-4 gene was detected in 1 strain Shewanella putrefaciens isolate obtained from the lake. The environment is the most important source and way of spreading this type of resistance in the Czech Republic.
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Solvency II: solvency in insurance
Čáha, Pavel ; Cipra, Tomáš (advisor) ; Mazurová, Lucie (referee)
This thesis is dedicated to Solvency II, a regulatory framework for insurance and reinsurance companies effective in European Union. Firstly, it explains the notion solvency and also describes the principles of the regulation itself. Another part is focused on the calculation of solvency capital requirement and minimal capital requirement, using standard formula. The capital requirements are derived on the level of risk modules and their submodules. Furthermore, the topic of technical reserves is discussed and emphasis is placed on the derivation of mean square error of prediction. Described methods are Chain-Ladder and Bornhuetter-Ferguson. The last part of the thesis includes the calculation of capital requirements for real data. A program SolvencyII.xlsx that shows particular derivations is enclosed.
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Using reverberators to modify space acoustics
Pavlikovský, Vladislav ; Orlovský, Kristián (referee) ; Kurc, David (advisor)
This diploma thesis deals with adjusting the reverberation time of enclosed spaces. It is divided into two thematic areas. The first thematic area deals with active systems that adjust the reverberation time, with a stronger focus on usage of reverberators to simulate secondary spaces. The second thematic area is the implementation of reverberators and their fundamental building blocks in Matlab.
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IrAM9 - a member of a thioester-containing protein family from the hard tick \kur{Ixodes ricinus}
RATHNER, Petr
The thioester-containing proteins (proteins of ?2-macroglobulin family) are constituents of innate immunity, which comprise (i) universal protease inhibitors of ?2M type, (ii) C3, C4, C5 components of the complement system, (iii) insect thioester-contaning proteins (iTEPs) and (iv) macroglobulin complement related proteins (MCR). In this work, the partial structure of the thioester-containing protein from the hard tick Ixodes ricinus (IrAM9) which belong to the MCR was determined by sequencing and cloning of PCR products. The IrAM9 message is expressed in the ovaries and salivary glands of partially engorged females. Received recombinant protein was used to get polyclonal serum which was obtained by repeated rabbit immunization. The obtained antibodies were used in an attempt to detect the native protein in tick tissues by immunoblotting method.
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